Inhibitory Effect Mediated by Deoxynivalenol on Rumen Fermentation under High-Forage Substrate

Deoxynivalenol (DON) is a type B trichothecene mycotoxin produced by Fusarium fungi. To investigate its ruminal degradability and effect on rumen fermentation, 2 × 5 factorial experiment was conducted in vitro with two feed substrates different forage levels (high (HF), forage-to-concentrate = 4:1; low (LF), 1:4) five DON additions per substrate (0, 5, 10, 15, 20 mg/kg of dry matter). After 48 h incubation, the HF group higher than LF (p < 0.01), it decreased along increase concentrations which varied from 57.18% to 29.01% at h. In addition, gas production rate, total VFA microbial crude protein linearly against 0.05). Meanwhile, proportion CH4 fermentation end-products increased linearly, especially 0.01). brief, microorganisms presented 29–57% degradation ability were particularly significant under high-forage substrate. Along increasing toxin decreased, showing dose-dependent response. However, inhibited methane when exceeded matter.